华谱科仪(北京)科技有限公司

Homepage | Favorite | 中文 | English


400-650-3365

GO



Your position: 华谱科仪 »Home »Products »HPLC Columns »S Series » S-Alkaloid

S-Alkaloid

  1. Description
  2. Feature
  3. Application
  4. Order
  5. Download

Alkaloids are one kind of important chemical compositions that have widely distributed in nature. And they usually possess significant special activity. According to published report, hundreds of drugs have been discovered,which are based on alkaloids as the guideline.To date, alkaloids are still the most potential natural products in drug discovery, and purification of alkaloids remains a hotspot of natural products research.

For the analysis of related impurities in pharmaceutical products it is particularly serious, since the peak shapes of bases always become worse with the injection amount increased. When the peak tailing become worse, the resolution between adjacent peaks will be poorer or even difficult to be identified because small peaks eluting close to the overloaded peak are swamped.It has long been recognized that the effective chromatographic separation of alkaloids is a huge challenge, because the interactions of positively charged amines of alkaloids with the negative silanols groups or potential trace metal impurity of silica gel that has widely beed used in high performance liquid chromaotgrpahy. These will lead to severe tailing of chromatographic peaks, poor selectivity and difficulty in the control of retention. And it will be more serious if large volume of sample injected. The peak shapes for separation of basic compounds can be improved by the use of high-purity silica, high bonding density, superior endcapped technology and high pH tolerant column. However, these methods could not address the peak tailing of bases completely, especially in the condition of large injection volumn when compounds purification or trace purities detecting.

Based on long-term experience and innovative technology, Acchrom has developed various chromatographic materials with diverse functional groups that can be used both in analytical and preparative mode.

  • Compatible with pure aqueous phase
  • Good retention for polar compounds
  • Superior peak shape in low pH without salt and ion pair
  • High loading capacity

Polar-copolymerized Technology

Based on the unique polar-copolymerized technology, Acchrom has developed a series of selective columns for alkaloids named S-Alkaloid columns via the strategy of introducing polar groups onto the traditional reversed phase materials. The introduction of polar groups reduces the redsidual silanol groups on the silica gel and shield the ionic interaction between ionized alkaloids and the residual silanol groups as well. Thus, superior peak shapes of bases can be obtained on S-Alkaloid columns. Another favorable feature of S-Alkaloid column is its high aqueous tolerance relative to C18 material, so it is easy to separate hydrophilic alkaloids.

Superior Peak Shape and High Loading Capability for Basic Compounds
Large injection volume is usually needed for the purification of impurities in pharmaceutical industry due to its extreme low concentration. On the S-Alkaloids PCP-C18 columns, symmetric peak shape could be obtained even under the conditions of high mass loading. And it is significant for the determination and purification of impurities.

mg level of alkaloid sample preparation can be realized on the analytical column leading to favorable application for scale-up purification of alkaloids.

Excellent Stability and Longer Column Life
S-Alkaloid PCP-SCX has especially long lifetime owing to its special surface bonded technology. The peak shape and retention time can be kept well even after 1000 times uses for the separation of berberine analogs.

Product name Particle Size Pore Size Column Specification Part Number
PCP C18 5 μm 100 Å 2.1 mmx 150 mm 13190510215
      3.0 mmx 150 mm 13190510315
      4.6 mmx 150 mm 13190510515
      10.0 mmx 150 mm 13150511015
         
PCP SCX 5 μm 100 Å 2.1 mmx 150 mm 13510510215
      3.0 mmx 150 mm 13510510315
      4.6 mmx 150 mm 13510510515
      10.0 mmx 150 mm 13510511015
         
PCP WCX 5 μm 100 Å 2.1 mmx 150 mm 13550510215
      3.0 mmx 150 mm 13550510315
      4.6 mmx 150 mm 13550510515
      10.0 mmx 150 mm 13550511015